The Lichtman laboratory and Engert laboratory at Harvard University, in collaboration with the Connectomics team at Google, are releasing the Fish1 dataset and its companion paper. Fish1 is the comprehensive whole-brain connectomics dataset for the 7-day post-fertilization (7dpf) larval zebrafish, combining electron microscopy (EM) and confocal light microscopy (LM) from the same specimen.

The resulting EM dataset, acquired at a resolution of 4 nm × 4 nm × 30 nm, provides ultrastructural details such as synaptic connections and subcellular features. This volume includes 187,053 cell bodies encompassing the zebrafish brain, spinal cord and ganglia, with 26,915 vglut2a-positive and 14,510 gad1b-positive cells. The dataset is editable through a collaborative proofreading tool, CAVE, allowing the community to reconstruct neurons of interest, analyze their connectivity, and overlay neurotransmitter information to test their circuit models.

• Gallery of the dataset.
• Instructions and examples for programmatic access to the data and its reconstructed products
• Browsing the dataset requires a Google account. To request editing privileges for the Fish1 dataset, please fill out this form.
• Proofreading using CAVE, or Connectome Annotation Versioning Engine